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neonatal human fibroblasts  (Lifeline Cell Technology)
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Lifeline Cell Technology neonatal human fibroblasts
Staphylococcus epidermidis inhibits fibroblast senescence by inhibiting SASP secretion from UVB-irradiated keratinocytes. (A) Schematic representation of the experimental design. NHEKs were cultured to 90% confluence and then exposed to 10 mJ/cm 2 UVB for induction. Subsequently, 75μg/mL of ≤10kDa S. epi was added to coculture for 48 hours. The conditioned medium containing SASP factors was collected by centrifugation at 2, 000 rpm for 20 minutes, mixed with fresh DMEM at a 1:2 ratio, and the final serum concentration was adjusted to 10%. The mixed medium was used to culture primary human <t>fibroblasts</t> for 48 hours. (B) Fibroblasts were stained with senescence-associated β-gal, and the percentage of senescent cells was quantified by Image (J, C) Protein levels of P16 and P21 in fibroblasts were analyzed by western blotting. Densitometric analysis of protein bands were quantified by Image (J, D) RT-PCR analysis of RNA isolated from fibroblasts was performed to assess the expression of P16, P21 , P53 , TNFα , IL-6 , IL-1β , and MMP1 , with β-actin as the internal control. Con-SASP, SASP collected from control NHEKs; S.epi -SASP, SASP collected from NHEKs treated with 75μg/mL of ≤10kDa S.epi ; UVB-SASP, SASP collected from NHEKs treated with UVB; UVB+ S.epi -SASP, SASP collected from NHEKs treated with UVB and 75μg/mL of ≤10kDa S.epi . Data represent mean ± SEM with n = 3. All the experiments have been repeated three times. Statistical significances were analyzed by One-way ANOVA. ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Neonatal Human Fibroblasts, supplied by Lifeline Cell Technology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neonatal human fibroblasts - by Bioz Stars, 2026-10
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Staphylococcus epidermidis inhibits fibroblast senescence by inhibiting SASP secretion from UVB-irradiated keratinocytes. (A) Schematic representation of the experimental design. NHEKs were cultured to 90% confluence and then exposed to 10 mJ/cm 2 UVB for induction. Subsequently, 75μg/mL of ≤10kDa S. epi was added to coculture for 48 hours. The conditioned medium containing SASP factors was collected by centrifugation at 2, 000 rpm for 20 minutes, mixed with fresh DMEM at a 1:2 ratio, and the final serum concentration was adjusted to 10%. The mixed medium was used to culture primary human fibroblasts for 48 hours. (B) Fibroblasts were stained with senescence-associated β-gal, and the percentage of senescent cells was quantified by Image (J, C) Protein levels of P16 and P21 in fibroblasts were analyzed by western blotting. Densitometric analysis of protein bands were quantified by Image (J, D) RT-PCR analysis of RNA isolated from fibroblasts was performed to assess the expression of P16, P21 , P53 , TNFα , IL-6 , IL-1β , and MMP1 , with β-actin as the internal control. Con-SASP, SASP collected from control NHEKs; S.epi -SASP, SASP collected from NHEKs treated with 75μg/mL of ≤10kDa S.epi ; UVB-SASP, SASP collected from NHEKs treated with UVB; UVB+ S.epi -SASP, SASP collected from NHEKs treated with UVB and 75μg/mL of ≤10kDa S.epi . Data represent mean ± SEM with n = 3. All the experiments have been repeated three times. Statistical significances were analyzed by One-way ANOVA. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Frontiers in Immunology

Article Title: Staphylococcus epidermidis prevents UV-induced skin aging by suppressing TLR3-mediated senescence

doi: 10.3389/fimmu.2026.1796085

Figure Lengend Snippet: Staphylococcus epidermidis inhibits fibroblast senescence by inhibiting SASP secretion from UVB-irradiated keratinocytes. (A) Schematic representation of the experimental design. NHEKs were cultured to 90% confluence and then exposed to 10 mJ/cm 2 UVB for induction. Subsequently, 75μg/mL of ≤10kDa S. epi was added to coculture for 48 hours. The conditioned medium containing SASP factors was collected by centrifugation at 2, 000 rpm for 20 minutes, mixed with fresh DMEM at a 1:2 ratio, and the final serum concentration was adjusted to 10%. The mixed medium was used to culture primary human fibroblasts for 48 hours. (B) Fibroblasts were stained with senescence-associated β-gal, and the percentage of senescent cells was quantified by Image (J, C) Protein levels of P16 and P21 in fibroblasts were analyzed by western blotting. Densitometric analysis of protein bands were quantified by Image (J, D) RT-PCR analysis of RNA isolated from fibroblasts was performed to assess the expression of P16, P21 , P53 , TNFα , IL-6 , IL-1β , and MMP1 , with β-actin as the internal control. Con-SASP, SASP collected from control NHEKs; S.epi -SASP, SASP collected from NHEKs treated with 75μg/mL of ≤10kDa S.epi ; UVB-SASP, SASP collected from NHEKs treated with UVB; UVB+ S.epi -SASP, SASP collected from NHEKs treated with UVB and 75μg/mL of ≤10kDa S.epi . Data represent mean ± SEM with n = 3. All the experiments have been repeated three times. Statistical significances were analyzed by One-way ANOVA. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Neonatal human epidermal keratinocytes (NHEKs) and neonatal human fibroblasts were purchased from Lifeline Cell Technology.

Techniques: Irradiation, Cell Culture, Centrifugation, Concentration Assay, Staining, Western Blot, Reverse Transcription Polymerase Chain Reaction, Isolation, Expressing, Control